My current research aims to provide an integrated geochemical and geomicrobial characterization of the freshwater microbial carbonate deposits actively forming in the prairie lowlands of the Everglades National Park. This characterization will integrate depositional fabrics and textural features of the marl sediments with next-generation 16S rRNA microbial surveys and pore water geochemistry. This integrative approach will make it possible to definitively assess the microbial pedigree of the primary deposits, as well as evaluate the subsequent diagenetic alteration. This characterization will be used as a calibration to examine ancient (Pleistocene) microbial carbonate deposits in the shallow subsurface of south Florida. Together, this modern and near-modern characterization of the depositional and diagenetic processes surrounding freshwater carbonate formation will address the longstanding questions of how microbial deposits are identified in ancient rocks, and the paleoenvironmental interpretation of microbial carbonate deposits found throughout geologic time.